[Association between serum lipoprotein-cholesterol levels and HDL subpopulations].

OBJECTIVE: To investigate the influence of serum HDL-C or LDL-C levels on the components of serum HDL subpopulations. METHODS: Apolipoprotein (apo) A-I contents of serum HDL subpopulations in 292 subjects were determined by two-dimensional gel electrophoresis in conjunction with immunodection. RESULTS: With the decrease of serum HDL-C levels, the apoA-I contents of pre beta1-HDL and HDL3b increased and were significantly higher (P<0.01; P<0.05) in the low HDL-C group than in the high HDL-C group. But on the contrary, with the decrease of serum HDL-C levels, the apoA-I contents of HDL2b and HDL2a decreased and were significantly lower (P<0.01) in the middle and low HDL-C groups than in the high HDL-C group. With the increase of serum LDL-C levels, the apoA-I contents of pre beta1-HDL, HDL3C and HDL3b increased; the apoA-I contents of pre beta1-HDL and HDL3b were significantly higher in the high LDL-C group (P<0.05) and very high LDL-C group (P<0.01), and those of HDL3C were also significantly higher in the very high LDL-C group (P<0.01). But on the contrary, with the increase of LDL-C levels, the apoA-I contents of HDL2b decreased and were significantly lower in the high LDL-C group (P<0.05) and very high LDL-C group (P<0.01) when compared with the apoA-I contents of the desirable LDL-C group. CONCLUSION: With the decrease of serum HDL-C levels or increase of serum LDL-C levels, the small-sized HDL increased and the large-sized HDL decreased; furthermore, the HDL-C levels were more closely related to the components of the large-sized HDL (HDL2a, HDL2b).

[The interrelationships between serum triglyceride, total cholesterol and HDL subpopulations].

OBJECTIVE: To assess the influence of serum triglyceride (TG) level and total cholesterol (TC) level on the change of the contents of serum HDL subpopulations. METHODS: The apolipoprotein (apo) A-I contents of serum HDL subpopulations in 289 subjects were determined by two-dimensional gel electrophoresis associated with immunodetection method. RESULTS: Analysis of the data on the serum TG levels in subjects revealed that the apoA-I contents of pre beta 1-HDL, pre beta 2-HDL, HDL3b and HDL3a increased with the increase of TG level, whereas the apoA-I contents of HDL2a and HDL2b decreased. By comparison with the data of normal TG group, the apoA-I contents of pre beta 1-HDL and HDL3b in the high TG and very high TG groups were significantly higher, whereas those of HDL2b were significantly lower in the high TG and very high TG groups. Analysis of the data on the serum TC levels in subjects revealed that the apoA-I contents of pre beta 1-HDL, pre beta 2-HDL, HDL2c and HDL3b increased with the increase of TC level, while the apoA-I contents of HDL2b decreased. As compared with the data of TC desirable group, the apoA-I contents of pre beta 1-HDL, HDL3c and HDL3b were significantly higher in the high TC group. CONCLUSION: With the increase of serum TG or TC, there is a general shift toward small-sized HDL in subjects. Besides, the change of serum TG level is a more important factor influencing the components of HDL subpopulations, compared with the change of serum TC level.